Methylation-specific PCR (MS-PCR or MSP) is one of the most commonly used methods for gene/sequence-specific detection of DNA methylation. The DNA undergoes bisulfite conversion of cytosine to uracil and then the methylated sequences are selectively amplified with primers specific for methylation. EpiGentek’s MS-PCR service specifically detects methylation in certain genes of cells and is especially useful for understanding the role DNA methylation plays in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Methylation-specific PCR approach to detect and
identify methylated status in CpG islands within one or
several gene targets
Everything included from sample preparation to data analysis
Accurate readout of methylation in targeted region
4 - 8 weeks from sample submission*
Included custom primer design for maximized amplification potential
Complimentary high-quality support to assist with data analysis at no additional charge
*Time measured when pre-payment for service is received
**Assuming 22 samples (with pre-isolated DNA and 1 primer pair submitted [1 methylated & 1 unmethylated]): may be subject to minimum spend
We currently accept DNA, cell, and tissue samples for our Methylation-Specific PCR (MS-PCR) service. Proper sample preparation along with the appropriate quality control methods on your end will allow the greatest chance for assay success. Please follow all guidelines for sample requirements, packaging and labeling, and shipping which are outlined on the Submission Instructions page.
Want more information on our Methylation-Specific PCR (MS-PCR) service or interested in customizing your research project? We’re here to help! Get in touch with one of our highly knowledgeable epigenetic services experts by calling toll-free at 1-877-374-4368 or emailing services @ epigentek.com.